Journal: Cells

Article Title: Loss of MER Tyrosine Kinase Attenuates Adipocyte Hypertrophy and Leads to Enhanced Thermogenesis in Mice Exposed to High-Fat Diet.

doi: 10.3390/cells13221902

Figure Lengend Snippet: Figure 4. TAM kinases are expressed in white adipocytes. (A) Relative gene expression levels of TAM kinases in the gWAT adipocytes of Mer+/+ and Mer−/−mice exposed to either ND or HFD determined by qRT-PCR at the end of the feeding experiment. (B) Changes in the mRNA expression levels of Mer during the differentiation of 3T3-L1 adipocytes. (C) Percentage and (D) images of 3T3-L1 adipocytes with large lipid droplets, after exposure for 3 days to BSA, to palmitate/oleate (150 µM each; 1× FA), or to (300 µM each; 2× FA) alone or in the presence of an inhibitory anti-Mer antibody or its isotype control. Scale bar, 100 µm. (E) Relative UCP1 mRNA expression in 3T3 adipocytes after 8 h exposure to 500 µM dbcAMP, to BSA, or to 1× FA alone or in the presence of the anti-Mer antibody or its isotype control. All the data are presented as mean and individual values, and statistical significance was evaluated by one-way ANOVA, except in A, where it was evaluated by two-way ANOVA. * denotes p ≤0.05, *** denotes p ≤0.001, and **** denotes p ≤0.0001.

Article Snippet: The cells were kept alone or treated with 10 nM inhibitory anti-mouse Mer antibody or with 10 μM pan-TAM kinase inhibitor BMS 777607 (Cat# HY12076, MCE, Monmouth Junction, NJ, USA) alone or with 10 μM forskolin, an adenylate cyclase activator (sc-3562; Santa Cruz Biotechnologies, Dallas, TX, USA).

Techniques: Gene Expression, Quantitative RT-PCR, Expressing, Control

Journal: Reproductive Sciences

Article Title: Female Infertility and Risk for Later-Life Cardiovascular Disease: Lessons from a Mouse Model of Human Cardiovascular Disease

doi: 10.1007/s43032-025-02026-y

Figure Lengend Snippet: Immunohistochemical evaluation of uteri on day 4.5 post-coitus in SR-BI KO/ ApoeR61 h/h mice. Leptin receptor (Leptin R) ( a-c ), cyclooxygenase (COX) −2 ( d-f ), leukaemia inhibitory factor (LIF) ( g-i ) and phospho-signal transducer and activator of transcription (Stat) −3 ( j-l ) immunostaining with haematoxylin counterstaining in SR-BI KO/ ApoeR61 h/h mice with placebo ( b , e , h , k ) or probucol treatment ( c , f , i , l ). As a control, uteri from wild type mice ( a , d , g , j ) were evaluated. Scale bar = 100 µm

Article Snippet: The 5-μm paraffin-embedded tissue sections were immunostained using monoclonal rabbit anti-mouse phosphorylated signal transducer and activator of transcription-3 (p-Stat3) antibody (Tyr705) (#9145; Cell Signaling Technology, Tokyo, Japan) at 1:400 dilution, polyclonal rabbit anti-mouse leukaemia inhibitory factor (LIF) antibody (OABF00432; Aviva Systems biology, CA, US) at 1:400 dilution, polyclonal goat anti-mouse leptin receptor antibody (AF497; R&D Systems, MN, US) at 1:200 dilution, and polyclonal rabbit anti-mouse cyclooxygenase (COX) −2 antibody (#160,126; Cayman Chemical, MI, US) at 1:500 dilution according to the manufacturers’ instructions.

Techniques: Immunohistochemical staining, Immunostaining, Control

Journal: Experimental and molecular pathology

Article Title: Expression of free fatty acid receptor 2 in normal and neoplastic tissues.

doi: 10.1016/j.yexmp.2024.104902

Figure Lengend Snippet: Fig. 7. Double-labelling immunohistochemical analysis of free fatty acid receptor 2 (FFAR2) expression and the expression of gastric inhibitory polypeptide (GIP; A–C) in the human duodenum, glucagon-like peptide 1 (GLP-1) in the human jejunum (D–F), or peptide YY in the human duodenum (G–I). Labelling of FFAR2 was visualised using Cy3-conjugated goat anti-rabbit antibody (red). Labelling of GIP, GLP-1, or peptide YY was visualised using Alexa Fluor 488-conjugated donkey anti- mouse antibody. Overlapping expression is represented by orange/yellow colour. Blue colour represents 4′,6-diamidino-2-phenylindole (DAPI)-stained DNA. Scale bar, 100 μm (A–I). Arrows in (A–C) indicate an enteroendocrine cell expressing both FFAR2 and GIP; arrowheads in (A–C) indicate an FFAR2-positive and GIP- negative enteroendocrine cell; arrows in (D–F) indicate GLP-1-positive and FFAR2-negative enteroendocrine cells; arrows in (G–I) indicate enteroendocrine cells expressing only FFAR2; and arrowheads in (G–I) indicate enteroendocrine cells expressing only peptide YY. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: For double-labelling fluorescence immunohistochemistry, sections were incubated overnight at 4 ◦C with rabbit anti-FFAR2 0524 antibody (1:100 dilution) together with mouse monoclonal anti-insulin antibody (1:100 dilution; Abcam), mouse monoclonal anti-glucagon antibody (1:500 dilution; Sigma-Aldrich, St. Louis, MO, USA), rat monoclonal anti-somatostatin-14/28 antibody (1:300 dilution; Abcam, Cambridge, UK), guinea pig polyclonal anti-gastrin antibody (1:800 dilution; Origene, Rockville, MD, USA), rat monoclonal anti-ghrelin antibody (1:50; R&D Systems/biotechne, Wiesbaden, Germany), mouse monoclonal anti-serotonin antibody (1:50 dilution; DAKO, Glostrup, Denmark), mouse monoclonal anti-gastric inhibitory polypeptide (GIP) antibody (1:1500 dilution; Invitrogen, Waltham, MA, USA), mouse monoclonal anti-glucagon-like peptide-1 (GLP-1) antibody (1:500 dilution; Santa Cruz Biotechnology, Dallas, TX, USA), or mouse monoclonal antipeptide YY antibody (1:100 dilution; Novus Biologicals/biotechne, Wiesbaden, Germany).

Techniques: Immunohistochemical staining, Expressing, Staining